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The effects of NaCl concentration and pH on the stability of hyperthermophilic protein Ssh10b

Background Hyperthermophiles constitute a group of microorganisms with an optimum growth temperature of between 80??C and 100??C. Although the molecular...

Stars: 121 Updated: September 7th,2018

Increased expression of Toll‐like receptor (TLR) 2 and TLR4 in the colonic mucosa of children with inflammatory bowel disease

0·1M Tris-HCl (pH = 8·0), 1mM ethylene glycol-bis (2-aminoethylether),N,N,N′,N′-tetraacetic acid (EGTA), 5 mM NaF, 1mM phenylme...

Stars: 165 Updated: September 7th,2018

Plant-induced changes in the rhizosphere of rape (Brassica napus var. Emerald) seedlings. III. Changes in L value, soil phosphate fr...

into the following forms: that extractable by an anion-exchange resin, 0.5 M NaHCO 3 (pH 85), 0.1M NaOH, 1m NaOH, 1mHCl and residual ...

Stars: 176 Updated: September 7th,2018

The adhesion molecule on glia (AMOG/beta 2) and alpha 1 subunits assemble to functional sodium pumps in Xenopus oocytes

buffer. Protein was eluted by incubation of the Sepharose beads with 0.5%...the eluate was adjusted to 8.2 by addition of 1M Tris/HCl, pH 7.0...

Stars: 155 Updated: September 7th,2018

CD spectra of purified L-asparaginase a) Far UV CD spectra of L-asparaginase at 0.1 mg/ml in 0.1M tris HCL (pH-8.4); b) Melting tem...

CD spectra of purified L-asparaginase a) Far UV CD spectra of L-asparaginase at 0.1 mg/ml in 0.1M tris HCL (pH-8.4); b) Melting temperature of enzyme (T222nm).Richi, V. Mahajan...

Stars: 174 Updated: September 7th,2018

TNF-Alpha Knockout and Minocycline Treatment Attenuates Blood Brain Barrier Leakage in MPTP-Treated Mice

Following intraparenchymal injection of the dopamine (DA) neurotoxin 6-hydroxydopamine, we previously demonstrated passage of fluoresceinisothiocyanate-...

Stars: 135 Updated: September 7th,2018

Purification and characterisation of xylitol dehydrogenase from Neurospora crassa NCL communication No. 6347

XD activity was determined spectrophotometrically at pH 8.4 and 28°C [9]. The assay mixture contained 0.1M Tris-HCl buffer, pH 8.4, 10 mM MgCl2...

Stars: 140 Updated: September 7th,2018

Đề kiểm tra 1 tiết bài 3 Hóa 12 (2012-2013) - THPT Trường Chinh

Đề kiểm tra 1 tiết bài số 3 môn Hóa học lớp 12 năm 2012-2013 của Sở Giáo dục và Đào tạo Ninh Thuận trường THPT Trường Chinh dành cho học sinh lớp 12 sẽ...

Stars: 98 Updated: September 7th,2018

The lipopolysaccharide-recognition mechanism in cells expressing TLR4 and CD14 but lacking MD-2

The agarose was washed three times with PBS containing 0.5% Nonidet P-40 and incubated with 0.1M glycine-HCl buffer, pH 2.5, for 5 min. The ...

Stars: 124 Updated: September 7th,2018

Primary structure and possible functions of a trypsin inhibitor of Bombyx mori

Tricine SDS/PAGE was performed according to Schägger and Von Jagow [11] on 16.5% polyacrylamide gel in 0.1m Tris/HCl buffer, pH 8.5, containing...

Stars: 101 Updated: September 7th,2018

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